Download Applied Tissue Engineering by Minoru Ueda (Ed.) PDF

By Minoru Ueda (Ed.)

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Additional control group, E: PRP-injected side; F: center of the distraction zone on the PRP-injected side; G: saline solution-injected side; H: center of the distraction zone on the saline solution-injected side (From Kinoshita et al. 2008. Reprinted with permission). Bone regeneration using ‘periosteum’ The periosteum is comprised of two tissue layers: the outer fibroblast layer that provides attachment to soft tissue, and the inner cambial region that contains a pool of undifferentiated mesenchymal cells, which support bone formation [24].

A B C D Fig. 22. Serial computed tomograms slicing the middle level of the alveolar cleft. A: Preoperation. B: Three months postoperation. Dome-shaped radiopaque images facing together and extending from the cleft bony walls inside the cavity. C: Six months postoperation. Fused image in the cleft cavity. D: Nine months postoperation. The lateral and supernumerary incisors are approximated in the bony bridge lateral to the reconstructed incisive canal (From Hibi et al. 2006. Reprinted with permission).

Recently, studies have reported the existence of osteogenic progenitors, similar to MSCs, in the periosteum [25, 26]. Under the appropriate culture conditions, periosteal cells secrete extracellular matrix and form a membranous structure [27]. The periosteum can be easily harvested from the patient’s own oral cavity, where the resulting donor site wound is invisible. Owing to the above reasons, the periosteum offers a rich cell source for bone tissue engineering. Chapter 3: Bone 27 Our group has previously demonstrated bone regeneration using a cultured periosteum (CP) in a critical-sized rat calvaria bone defect [28] (Fig.

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