By Donna H. Wang
Michigan country Univ., East Lansing. interprets wisdom into reproducible tools than allow biomedical researchers to discover the function of angiotensin in future health and illness. Covers genetic manipulation of special genes. comprises tools for practical experiences of the renin-angiotensin procedure. DNLM: Renin - Angiotensin System-physiology
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In the presence of cre-recombinase, the STOP signal is deleted and expression of the construct is induced. Gene activation via cre-recombinase also forms the basis for a variety of cre-reporter systems that have been recently developed to provide a rapid and easy screen for cell-specific expression and function of cre-recombinase. In one example, expression of a floxed `-gal gene is under the control of a ubiquitous promoter that is active in all cell types, and expressed both throughout development and in adults (19).
Collecting embryos: Around 56 h postcoitum, females with a plug are sacrificed and the abdominal cavity is opened up. The upper end of an uterine horn is grasped with forceps and gently lifted up. The mesometrium attached is gently removed and the uterus at about 5 mm from the oviduct-uterus junction and the portion between the oviduct and the ovary are dissected. The dissected oviducts are temporally stored in M2 medium in a 35-mm dish. Under a dissecting microscope, the oviduct is placed on an empty 35-mm dish.
J. Physiol. 272, F299–304. 6. , Labosky, P. , et al. (1995) Effects on blood pressure and exploratory behaviour of mice lacking angiotensin II type-2 receptor. Nature 377, 748–750. 7. Esther, C. , Howard T. , Marino, E. , Goddard, J. , Capecchi, M. , and Bernstein, K. E. (1996) Mice lacking angiotensin-converting enzyme have low blood pressure, renal pathology, and reduced male fertility. Lab. Invest. 74, 953–965. 8. , Marth, J. , Orban, P. , and Rajewsky, K. (1994) Deletion of DNA polymerase ` gene segment in T cells using cell type-specific gene targeting.